After the successful induction of experimental diabetes, the rats were divided into five groups each comprising a minimum of six rats. The dna sensor aim2 protects against streptozotocin. It is antidiabetic and hypolipidemic activity ofgymnema estimated that diabetes would affect approximately sylvestreaqueous leaf extract on both normal and alloxan 57 million people by the year 2025 2. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. Effect of potentilla fulgens on lipid peroxidation and. Nicotinamide protected nmri mice against alloxan induced hyperglycemia when given 10 min before, but not 10 min after, the injection of the drug. The antidiabetic activity of the total lignan from the plant fructus arctii, used in china for the control of diabetes, was investigated in models of alloxan. The present study evaluates the effects of chitooligosaccharides cos for the management of alloxan induced diabetes in mice. Cinnamon administered group show restoration in both biochemical and histol ogical parameters of kidney of mice.
Niclosamide ethanolamineinduced mild mitochondrial. Intramuscular or intraperitoneal injection of a single alloxan dose 300 mgkg is shown to cause a temporary, modest rise in blood glucose levels in the chinese hamster boquist, 1967. The antidiabetic activity of total lignan fromfructus arctii. Streptozotocin stzinduced diabetic animal models are useful. In this study, ethyl acetate fraction scee of sida cordata was investigated for scientific validation of its folk use in diabetes. The best known druginduced diabetes model is the alloxan diabetes. Type 2 diabetes t2d has reached an epidemic level globally. Optimization of alloxan dose is essential to induce stable diabetes for prolonged period alloxan dose for experimental induction of diabetes in rats has to be optimized.
In addition, it was reported that very high lipid levels can be induced in obese zucker diabetic fatty zdf rats, using high saturated fat and. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. The greater the dose, the less mice accepted the tumor. Hence, it is possible that other ligandreceptor pairs control a later phase of disease. Alloxan induced diabetes in mice protocol diabetestalk. Streptozotocininduced diabetic models in mice and rats. Lower doses can result in autoreversion to normal state without medical help, while higher doses cause toxicity and loss of experimental animals. The antidiabetic activity of total lignan from fructus. Cinnamon extract on alloxan induced diabetes in experimental mice. For the management of the carbohydrate metabolism in diabetes by the cos, the amount of glucose in blood along with. This study describes a rat diabetes mellitus model induced by administering a reduced dose of alloxan, thus greatly reducing the animals death rate. Antidiabetic evaluations of some traditional plants in. Antioxidant and hypoglycemic effects of tithonia diversifolia aqueous leaves extract in alloxan induced diabetic mice abstract diabetes is a chronic disease cause the metabolic disorder and associated with serious complications. The mechanisms of alloxan and streptozotocininduced.
One group of eight normal mice was kept as a control. How to induce diabetes in rats with alloxan diabetestalk. Potentilla fulgens rosaceae root traditionally used as a folk remedy by local health practitioners of khasi hills, meghalaya was investigated for its effects on lipid peroxidation and antioxidant status in alloxan induced diabetic mice. Prevention of diabetes in nod mice at a late stage by.
Antidiabetic effect of ethanolic leaf extract of phyllanthus. Palliative effects of daonil supplemented with tryptophan on. Previously it has been reported that up780, a standardized composition of aloe chromone formulated with an aloe polysaccharide, has a significant impact in reducing hba1c, fasting blood glucose, fructosamine and plasma insulin level in humans and improved impaired glucose and insulin. The mechanism of alloxan and streptozotocin action in b cells. The treatment effects were compared with the normal control, diabetic control, and diabetic. However, others workers reported its different doses for induction of diabetes. Experimental diabetes mellitus in different animal models ncbi. A study was undertaken to evaluate the effects of polysaccharide from lycium barbarum lbp in alloxan induced diabetic mice. Phytochemical screening and antidiabetic effect of. Rats were made to fast prior to alloxan administration. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. This study investigated the protective effect of a liquid rice hull smoke extract rhse against diabetes in alloxan induced diabetic mice.
In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizona and antiinsulin serum. Diabetes mellitus is the most important non infective epidemic to hit the globe in the present millennium. Science and technology, general diabetes care and treatment diabetes mellitus gooseberries health aspects. Therefore, this study was designed to systematically compare the influence of selenite treatment on gsh levels in blood and tissues such as brain, kidney, liver, spleen and testis in kunming mice with diabetes induced by alloxan. Alloxaninduced diabetes model diabetes was induced by a single intraperitoneal injection of 150 mgkg monohydrated alloxan sigma, st. Alloxan, a pyrimidine nucleic acid, has a cytotoxic effect on cells and has been used to induce diabetes in the chinese hamster. After 21 days of treatment it was found that the synergistic effect of mh with turmeric was greater in reducing the blood glucose level 143. We hypothesized that nox2 deficiency decreases reactive oxygen species ros production and immune response and protects against streptozotocin stz induced. Pulmonary function reduction in diabetes with and without chronic obstructive. Alloxaninduced diabetes triggers the development of. In 1838, wohler and liebig 1 synthesised a pyrimidine derivative, which they later called alloxan 2, 3. Various studies have shown that diabetes mellitus is associated with increased the formation of free radicals and. Role of aopioid receptors in formalin induced pain behavior in mice chengshui zhao,a yuanxiang tao,a jill m. Effect of shilajit on blood glucose and lipid profile in.
Growth and treatment of ehrlich tumor in mice with alloxan. Pdf antidiabetic potential of caralluma europaea against. Diabetes division, baker idi heart and diabetes institute, melbourne, australia. Diabetes was induced with streptozotocin stz, 60 mgkg, i. Diabetes mellitus is a potentially morbid condition with high prevalence worldwide, thus being a major medical concern. The resulting insulinopenia causes a state of experimental diabetes mellitus called alloxan diabetes 4, 8, 9. Trigonelline treated group showed islet cells in the vicinity of the pancreatic duct which indicates its. Mice with acute diabetes induced by stz were administered metformin by gavage. Effect of aspergillus awamorifermented burdock root on. The antidiabetic activity of the total lignan from the plant fructus arctii, used in china for the control of diabetes, was investigated in models of alloxan induced diabetic mice and.
As existing treatment is inadequate, research for better drugs should be carried out. Effect of honey and food supplements turmeric, lemon, ginger and garlic either alone or in respective combinations were tested on alloxan induced diabetic mice. Alloxan and streptozotocin are widely used to induce experimental diabetes in animals. Department of zoology, sadguru gadage maharaj college, karad 415110, india available online. Only animals with clinical signs of severe diabetes and fasting glucose. Pretreatment in vivo with nicotinamide induced hyperglycemia at the time of alloxan injection, and this could account for the protective action of nicotinamide against alloxan diabetes. Then, alloxan 200 mgkg of body weight was administrated to each mouse.
Alloxaninduced diabetes delays repair in a rat model of. Alloxaninduced diabetes, a common model for evaluating. A correlative study with antihyperlipidemic and antioxidative activity. All over the whole world, mushrooms have been cherished for thousands of. Jun 25, 2009 stz and alloxan induced diabetic mice. Dec 18, 2007 alloxan has two distinct pathological effects. Alloxaninduced diabetes causes morphological and ultrastructural changes in rat liver that resemble the natural history of chronic fatty liver disease in humans. Blood glucose levels and body weights of control and diabetic mice were monitored. After 48 h, the animals showing serum glucose levels.
Group 1 was the normal control mice that received distilled water as placebo while groups 26 were diabetic mice. Such effect was concomitant with an enhanced histological fea tures of pancreatic lesions, leading to a possible pancreato. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. We found that mice with alloxan induced diabetes had lower gsh levels in blood, kidney, liver and testis compared. Stz induced diabetes represents an immunemediated mouse model of t1d.
A total of 24 and 72 adult albino rats were used for the acute 24 hours and subchronic 15. In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and oxidative stress status of the prefrontal cortex. Alloxan treatment 10 mm increased cellular reactive oxygen species ros levels in the. Hnf1a, 1b, and 4a isoforms genes directly linked to maturity onset diabetes of the young mody in humans do not emu. Jan 04, 20 please use one of the following formats to cite this article in your essay, paper or report. Stz can be administered as multiple low doses over 5 days to induce insulitis in mice like and rossini, 1976. Differential effect of honey on selected variables in alloxan. Nox2 deficiency protects against streptozotocininduced. Efficacy of chitooligosaccharides for the management of diabetes in alloxan induced mice. The present study aimed to investigate the potential antidiabetic and hypolipidemic activity of tea polysaccharides in mice with alloxan induced diabetes.
Effect of oral administration of lactobacillus casei on. Animals models for studying diabetes mellitus etuk, e. The use of animal models in diabetes research ncbi nih. Research article alloxan induced diabetes mellitus in wistar. Effect of alloxaninduced diabetes mellitus and ethanol on. Antioxidant and hypoglycemic activity of strawberry fruit extracts against alloxan induced diabetes in rats sheriff sheik abdulazeez1 and ponmurugan ponnusamy2 1college of applied medical sciences, shaqra, shaqra university, kingdom of saudi arabia 2ks rangasamy college of technology, thiruchengode, tamil nadu, india.
The cytotoxic action of both these diabetogenic agents is mediated by reactive oxygen species, however, the source of their generation is different in the case of alloxan and. Aljassabi, ali saad, mohd sofian azirun and anas alomari4 1faculty of medicine, masterskill university, jb, malaysia 2faculty of pharmacy, pharma d, just, jordan 3institute of biological sciences,university of malaya, malaysia. Diabetes was induced in rats by administration of alloxan 150 mgkg, i. Effect of aloe vera leaf extract on alloxan induced. As a thiol reagent, alloxan also selectively inhibits glucoseinduced insulin secretion through its ability to inhibit the beta cell glucose sensor glucokinase. Pdf the mechanism of alloxan and streptozotocin action. In research it is used for induction of type 1 diabetes. Antidiabetic effects of rhse were evaluated in both the rat insulinoma1 cell line ins1 and diabetic icr mice induced by intraperitoneal ip injection of alloxan. Antidiabetic effects of rice hull smoke extract in alloxan.
To study the effect of shilajit a herbomineral preparation on blood glucose and lipid profile in euglycemic and alloxan induced diabetic rats and its effects on the above parameters in. Antidiabetic and antioxidant effects of zingiber officinale. Mice fed the burdock diet and the control diet for 14 weeks. Alloxan is a urea derivative which causes selective necrosis of the. Effects of polysaccharide from lycium barbarum in alloxan. Blood glucose lowering activity of aloe based composition. A diet containing the 5% fermented burdock powers was prepared to examine effect of the burdock diet on alloxan induced mouse diabetes.
Role of aopioid receptors in formalininduced pain behavior. This causes an insulindependent diabetes mellitus called alloxan diabetes in these animals, with characteristics similar to type 1 diabetes in humans. U department of pharmacology, college of health sciences, usmanu danfodiyo university, sokoto, nigera phone. May 16, 2011 in summary, huff and his colleagues demonstrated that, both in vitro in human hepatoma cells and in vivo in a mouse model of insulin resistance and atherosclerosis, nobiletin exhibits antiatherogenic and antidiabetic properties. A total of 24 male, hyperlipidemic, alloxan induced diabet ic mice were divided into three groups. Most current treatments ameliorate the hyperglycemic. Changes of blood glucose and body weight, and the daily. Type i diabetes mellitus was induced in mice by intraperitoneal administration of alloxan monohydrate followed by treatment with the therapeutic doses of the aqueous and ethyl acetate leaf extract of k africana to the experimentally diabetic mice. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. Experimental model of induction of diabetes mellitus in rats. Alloxan is selectively toxic to insulinproducing pancreatic beta cells because it preferentially accumulates in beta cells through uptake via the glut2 glucose transporter.
In conclusion we showed that icv injection of insulin in non diabetic rats, in contrast. Alloxan is routinely used intraperitonealy to induce diabetes in experimental animals and constitutes a simple tool for testing the antidiabetic potential or changes in analytes in the diabetic state. Nobiletin exhibits antiatherogenic and antidiabetic. Metformin ameliorates insulitis in stz induced diabetic mice peerj. Periodontal disease in diabetic patients presents higher severity and prevalence. Alloxan definition of alloxan by medical dictionary. These findings indicate that nobiletin may help mitigate human insulin resistance and the associated lipid. Mice inhaled radon at concentrations of, 2500, and 5500 bqm3 for 24 hours before stz administration. Pdf hypoglycemic effect of lagerstroemia speciosa l.
Stz administration induced characteristics of type1 diabetes such as hyperglycemia and hypoinsulinemia. Diabetes was induced using intravenous alloxan sigma co, usa at a dose of 42 mgkg of body weight into tail veins. The mechanisms of alloxan and streptozotocininduced diabetes. Type 3 diabetes has been suggested as a term for alzheimers. Sensitization to alloxan induced diabetes and pancreatic cell apopto. In vivo hypoglycemic effect of kigelia africana lam. Experimental models play an important role in understanding such a disease, which is treatable only. All the three peel extracts nearly reversed most of these changes induced by alloxan suggesting their possible role in ameliorating diabetes mellitus and related changes in serum lipids. It has been successfully induced in a variety of animal species. A comparative study on the role of omani honey with. Effect of aloe vera leaf extract on alloxan induced diabetes in young mice j.
Antidiabetic effect of sida cordata in alloxan induced. It has been widely used to induce experimental diabetes in animals such as rabbits, rats, mice and dogs with different. The various parameters studied included body weight, fasting blood glucose levels, total cholesterol tc and triglyceride tg in diabetic and normal mice. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. These agents selectively destroy the langerhans islet. Antidiabetic and hypolipidemic activity of gymnema sylvestre. Protective role of three vegetable peels in alloxan induced. The role of silymarin in prevention of alloxaninduced diabetes mellitus in balbc mice 1 23s. Sensitization to alloxaninduced diabetes and pancreatic cell apoptosis in acatalasemic mice article in biochimica et biophysica acta 18022.
Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Recently, padmanabhan and shafiullah 28 demonstrated an increase in resorptions, intrauterine growth restriction and congenital anomalies in the litters of to mice treated with streptozotocin induced diabetes mellitus receiving a single dose of ethanol on gestational day 7 or 8. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals as a result of the specific necrosis of the pancreatic beta cells 57. Antihyperglycemic effects of fermented and nonfermented. May 24, 2014 there are a few nutritional approaches to address the increased needs of managing diabetic conditions. Diabetes was induced using intraperitoneal injection of alloxan 100 mgkg, 0. Comparison of streptozotocin and alloxan induced diabetes in the rat, including volumetric quantitation of the pancreatic islets, volume 9, issue3, pp 178184 cite as comparison of streptozotocin and alloxan induced diabetes in the rat, including volumetric quantitation of the pancreatic islets diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. In nod mice, cd28b7 and cd40cd40 ligand l interactions control the onset of diabetes from 2 to 4 weeks of age, but blocking these molecules has little effect after this time. Postprandial antidiabetic effects of various fractions of fagonia indica burm. Dose optimization of alloxan for diabetes in albino mice. Rauwolfia serpentina ameliorates hyperglycemic, haematinic. In the present study activities of liver enzymes such as glucokinase, glucose 6 phosphatase and fructose 1 6diphosphatase were also determined. Chronic treatment of diabecon in the regulation of alloxan induced hyperglycemia and oxidative stress in different tissues of adolescent diabetic rats agrawal jyoti 1 and kar anand 1thyroid research unit, school of life sciences, devi ahilya university takshshila campus, indore 452017, m.
By the year 2025, india shall have the maximum number of diabetes in the world making it, the diabetes capital of the world despite the great strides. Effect of combined dyslipidemia and hyperglycemia on diabetic. Alloxaninduced diabetic mice, antidiabetic activity, astraeus hygrometricus. This study was designed to investigate the hypoglycaemic and antioxidant effects of zingiber officinale on experimentally induced diabetes mellitus using alloxan and insulin resistance. Suppression of streptozotocininduced type1 diabetes in. The results showed that acatalasemic mice developed more severe atrophy of pancreatic islets and apoptosis of islet cells and promoted diabetes in comparison to the wildtype mice in an alloxan induced experimental diabetes model. Stz and alloxan induced diabetic mice scientist solutions. Optimization of alloxan dose is essential to induce stable. Objective the role of nox2containing nadph oxidase in the development of diabetes is not fully understood. Report by journal of pure and applied microbiology. After oral administration of each mesc singly or repeatedly to alloxan.
Antidiabetic effect of scee was confirmed by antihyperglycemic activity in normal glucose loaded and diabetic glucose loaded animals as well as normal off. This study was conducted to compare the antidiabetic effect of. The mechanism of their action in b cells of the pancreas has been intensively investigated and now is quite well understood. Influence of alloxaninduced diabetes and selenite treatment. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Alloxan was injected into the rats through a tail artery and three days later, a confirmation of successful induction of diabetes was made by demonstration of hyperglycemia in the rats. The extract of bitter melon, chirata and cinnamon were tested for its efficacy in alloxan at a dose 120mgkg of body weight induced diabetic mice. Following its uptake into the beta cells, streptozotocin is split into its glucose and methylnitrosourea moiety. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Sensitization to alloxaninduced diabetes and pancreatic cell.
The role of silymarin in prevention of alloxaninduced. Insulin injection into diabetic tumorbearing mice promoted the tumor growth. Medicinal plants are efficient ameliorator of oxidative stress associated with diabetes mellitus. After 12 h, a 10% glucose solution was offered to the animals to prevent hypoglycemia 27,28. Research article alloxan induced diabetes mellitus in. Antidiabetic study of fjmk in alloxan induced diabetic model induction of experimental diabetes diabetes was induced in mice by a single injection of alloxan monohydrate 150 mg kg i. Pdf efficacy of chitooligosaccharides for the management. Pdf hypoglycemic activity of ethanolic extract of astraeus. Sensitization to alloxan induced diabetes and pancreatic cell apoptosis in acatalasemic mice yoko kikumoto, hitoshi sugiyama, tatsuyuki inoue, hiroshi morinaga, keiichi takiue, masashi kitagawa, naomi fukuoka, mizuho saeki, yohei maeshima, dahong wang, et al. It was observed that the mice given a diet containing 0. Trigonelline significantly reduced theblood glucoseintreated groupsascompared tocontrol diabeticanimals. In this study the effects of petroleum ether and ethanol extracts of the seeds of citrullus lanatus on blood glucose levels in alloxan induced in mice have been investigated.
After cancer and cardiovascular diseases, diabetes is the third most common cause of death. Alloxaninduced diabetes and bone repair 373 issn 0100. The mice were randomly assigned into six different groups 8. However, cucurbita pepo peel was found to be the most effective. The mechanisms of alloxan and streptozotocin induced diabetes.
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